They were revealed for fluorescent staining by Alexa 488- (Invitrogen, Carlsbad, CA) or by Cy3- or Cy5-labelled (Jackson Immunoresearch, Suffolk, UK) secondary antibodies of the appropriate specificity, for bright field observation by biotin-labeled secondary antibodies and Vectastain ABC kit (Vector Laboratories, Peterborough, UK) revealed with 3,3′-diaminobenzamide3,3′-diaminobenzamide
Permeation of 1 into cells and subsequent hydrolytic activation by CEs causes spontaneously quinone methide cleavage, resulting in bright red fluorescence in ER with high specificity.
Although TβRII antibodies showed specificity relative to IgG staining, this bright punctate staining is insufficient for visualizing fine structural organization.
Unfortunately this was the bright side of the story; on the other hand, phage specificity imposed a complicated dilemma, which is the difficulty of finding sufficient phage strains which could cover all or most of bacterial host strains.
What Midas shops offer is honesty, a certain specificity, and half-hour-while-you wait service in a bright, pleasant shop.
Bright dotted foci and hyperechoic stippled aggregates have 80% sensitivity and 75% specificity.
In San Francisco, where the afternoon was unusually bright and warm, strollers wandered near the bridges with just a little concern for the specificity of the warning Governor Davis cited, of terrorists striking one of the state's four suspension bridges between Nov. 2 and Nov. 7.
The presence of bright stippled foci and/or hyperechoic areas on US indicated gout with great sensitivity (96%), whereas the specificity is limited (73%).
Contrast agents (CAs) can improve the contrast and specificity in particular target regions of MR images, and these are widely used to produce brighter and darker areas with T1 and T2 CAs, respectively.
