Under bright field observation the membrane in the upstream region of some of the cells had detached from the cell cytoskeleton (Fig. 6).
They were revealed for fluorescent staining by Alexa 488- (Invitrogen, Carlsbad, CA) or by Cy3- or Cy5-labelled (Jackson Immunoresearch, Suffolk, UK) secondary antibodies of the appropriate specificity, for bright field observation by biotin-labeled secondary antibodies and Vectastain ABC kit (Vector Laboratories, Peterborough, UK) revealed with 3,3′-diaminobenzamide3,3′-diaminobenzamide
The most obvious feature of metallic stent struts is a bright reflection followed by a dark shadow, and strut detection approaches incorporate this observation and more.
Information of electron acceleration is obtained by hard X-ray, radio wave, and H α bright line observations.
Calcofluor staining and bright field observations revealed the presence of fungal mycelium in the tissues immediately adjacent to the autofluorescent regions (Figure 7S and T, respectively), indicating that autofluorescence was a genuine defence-associated response against leaf stripe.
In the scutellar node and basal region of provascular tissue of the inoculated sample we observed, on average, 500 cells per section and time point of inoculation that were in contact with the fungus (on the basis of the calcofluor staining and bright field observations) and only one to two nuclei were positive to TUNEL staining.
During the first 2 hr of SN oocytes culture, bright-field observations showed that the germinal vesicle break down (GVBD) occurs on average 34.2 ± 8.1 min since the starting of the recording (Table 1).
At the same time, their devices were not able to captivate variation in brightness, and our observation of bright spots on the fault is a new finding.
In the few cells that can technically be analyzed, the amount of IFT material is high, turning the flagellum very bright and preventing observation of individual trains (Slide 1, Film 49h).
