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Whole brains were frozen in isopentane, stored at 80°C and 15 µm thick sections were cut using a cryostat (Bright Instruments Ltd).
Coronal brain sections of 30 μm thickness were taken on a cryostat (Bright Instruments, UK).
Brains were frozen in Tissue-Tek OCT (Sakura Finetek) and sectioned with a cryostat (20 μm; Bright Instruments, Huntingdon, UK).
Muscle was sectioned into six‐micron sections using a cryostat (Bright Instruments Co., Huntingdon, UK) and used staining protocols described in the Supplemental Experimental Procedures.
Six μm thick cryostat (Bright Instruments, UK) sections were cut and fixed in acetone, and endogenous peroxidase activity was then blocked with 0.1% Hydrogen Peroxide.
Thirty μm thick coronal sections were cut using a sledge microtome (Bright Instruments, Cambridge, UK) and stored in cryoprotectant solution (30% ethylene glycol, 20% glycerol in PBS) at −20 °C until used.
Similar(48)
compound and rapidly frozen, and 14-μm sections were obtained using an OTF5000 cryostat (Bright Instrument Co. Ltd., Huntingdon, UK).
Tissue was embedded in Cryo-M-Bed (Bright Instrument, Huntingdon, Cambridgeshire, UK) and sectioned at 14 μm.
Frozen sections of 10 μm thickness were cut using a microtome cryostat system (Bright Instrument Co. 5030, Huntington, UK).
Frozen tumour specimens were made into 5 μm sections using a cryostat (Bright Instrument Company, Huntingdon, UK) and the sections transferred to RNALater (Ambion (Europe) Ltd ,Huntingdon, UK).
The embedded carcass was put on the quick freezing stage (−25°C) in the cryostat (Bright Instrument Company Ltd., UK) for about 120 minutes.
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