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The CT values show that the AuNS@PEG nanoparticles have a good bright imaging.
In accordance to [10] and Figure 7 (more bright imaging at the end of particles), we could suppose about the increase of the local electromagnetic field at the edges of the different graphene particles.
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It is difficult to observe hydrogel villi structures under the bright field imaging using a conventional microscope due to its bright color and transparency.
Images of whole tumour sections were captured (× 20 objective) using a Leica fluorescence microscopic imaging system (Leica Microsystems Inc ,Richmond Hill, ON, Canada) equipped with both cooled 350FX monochrome and 300FX colour cameras for fluorescence and bright field imaging, respectively, an automated scanning stage (DM6000B), and Surveyor software (Objective Imaging, Kansasville, WI, USA).
All images were recorded under uniform illumination in a bright field imaging mode, using sufficient magnification (×200).
Combined with simple bright field imaging, obviating the need for fluorescent labels or beads, we quantify and analyze key cardiac contractile parameters.
Dead cells are stained blue and display as dark cells that can be identified by the Cellometer software with bright field imaging.
The microstructure in the naturally aged condition was characterized using transmission electron microscopy techniques; phases present were identified by selected area diffraction, and dislocation morphology (helices vs loops) was examined by two-beam bright field imaging.
(B) Resected tumors were analyzed by bright field imaging (top row) and immunohistochemistry for human CD3 expression and dark field imaging (bottom row) to indicate the presence of AuNPs.
However, measuring the cluster size or validating the existence of cells in very small clusters (<1000 µm2) could not be properly determined using bright field imaging.
After removal of the staining solution and 3 PBS washes, the X-gal staining was allowed to intensify in PBS at 4°C before mounting and bright field imaging.
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