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The lure of the bright examination lights caught me, and I've been in the specialism for 10 years now.
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For bright field examination and correlated light and electron microscopy (EM), BDA was developed with avidin biotinylated horseradish peroxidase complex (ABC in TBS, 2 h, 1 300, Vector Laboratories) using Nickel-intensified diamino-benzidine (DAB-Ni, black precipitate) as a chromogene.
At 24 h after the wounding, cell migration was determined by bright-field microscopic examination.
Upon closer examination, the bright spots in Fig. 6 were found to be mainly zinc sulfide particles.
Let's be real: we'll either lie under the earth, decomposing like a compost pile with maggots nibbling at our flesh, or we'll be lying under the bright lights of an examination room.
Sections were counterstained with haematoxylin (Sigma-Aldrich, USA), dehydrated, cleared and coverslips were mounted prior to examination by bright field microscopy (Leica DM4000B, Leica Germany).
Rectal examination showed bright red blood, but no distinct mass.
Furthermore, the evidence for these abnormalities could not be obtained by examination of bright field images.
Coverslips were mounted onto slides with AquaPolyMount (Polysciences, Inc. Warrington, PA) for subsequent examination using bright field light microscopy.
After 1 min, the cervix was illuminated with a bright lamp and visually examined ('naked eye' examination).
A vaginal swab was mixed with 1 ml of saline and one drop was placed on a slide for examination under bright-field microscopy at × 200 400 magnification.
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