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Through fluorescence-activated cell sorting (FACS), we isolated the bright cells from the heterogenous population of C. beijerinckii cells expressing CbFbFP.
The purpose of this study is to find out if aldehyde dehydrogenase bright cells taken from a patient's bone marrow can be placed safely, via intramuscular injections, into their affected calf and lower thigh muscles and improve blood flow and/or peak walking time in patients experiencing pain associated with blocked blood vessels in the leg.
In c, dark cells: bacteria with the typical 'Candidatus Accumulibacter phosphatis' morphology; bright cells: bacteria with the typical 'Candidatus Competibacter phosphatis' morphology.
To reduce noise introduced by the presence of small numbers of very bright cells in the population, median fluorescence was used as the measure of population fluorescence intensities.
The remaining K8SP TECs in the medulla of DKK1 mice (see white arrows) were primarily the mature UEA1 bright cells (data not shown).
Phenotypic analyses of cytotoxic and helper subsets were performed placing an electronic gate on CD8+ bright and CD4+ bright cells and evaluating the expression of CD45RA versus CCR7.
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By mixing ADA-deficient and normal cells and using enzymatic amplification, we determined that our staining procedure could detect as little as 5% ADA-bright cells.
Aldehyde dehydrogenase-bright cells expressed CD34 or CD133 cell surface markers (57.0% and 27.1%, respectively), correlated closely with CD133+CD34+ cells (r = 0.72; p < 0.001), and differentiated into endothelial cells with greater efficiency than CD133+CD34+ cells.
We assessed the reproducibility of these estimates, correlation among EPC assays, and the association of ALDH(br) numbers with age and disease severity.Aldehyde dehydrogenase-bright cells were easily identified in nonmobilized peripheral blood with median and mean frequencies of 0.041% and 0.074%, respectively.
After several days in culture, a layer of stromal-like cells arose from adherent plated tissue (termed cardiac explants) over which small, round, phase-bright cells migrated.
CFSE-bright cells and CFSE-dull cells were mixed at a 1∶1 ratio, and then a total of 1×106 cells was injected i.v. into the indicated group of mice.
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