Briefly, solution A (100 mL) was mixed with solution B (2 mL) to prepare the analytical reagent.
Briefly, solution of Fenton's reagent [Fe (III) chloride, ascorbic acid and H2O2] was prepared in distilled water just prior to use.
(18) Briefly, solution calcium (0.5 mM), magnesium (0.5 mM), and potassium (0.15 mM) concentrations were maintained while alkalinity (NaHCO3) was varied between 0.5 and 6 meq/L in equilibrium with the atmosphere (pH 7.8 8.1).
Briefly, solutions of phospholipids, chloroform, and methanol were loaded into an autoclave.
Briefly, solutions of varying acrylamide and bis-acrylamide concentrations were polymerized by ammonium persulfate (10% w/v; 1/100 v/v) and tetramethylethylenediamine (1/1000 v/v; Bio-Rad; Hercules, CA).
Briefly, solutions containing seven distinct bacterial inoculum, ranging from 10 (0.5 McFarland standard) to 10 CFU/ml were prepared to facilitate bacterial counting in each plate.
Briefly, solutions containing 156 μM nitroblue tetrazolium (NBT) dissolved in 50 mM phosphate buffer (pH 7.4), 468 μM nicotinamide adenine dinucleotide (NADH) and various concentrations of extracts were mixed.
Briefly, solutions of (a) 0.7 M NaNO2, 0.7 M H2O2 and (b) 0.6 M HCl, were pumped using a syringe infusion pump (Harvard Apparatus, South Natick, MA) at 25 ml/min, into a Y-junction and mixed in a 2 mm-diameter by 0.5 cm silica tube.
Briefly, Peroxidase Block solution and Protein Block solution was applied on slides before the anti-PrP (1 500, 3F4) or anti-BiP (1 500, Cell Signaling Technologies, Danvers, MA, USA, #3177) antibodies.
22 Briefly, lysis solution was added to each well, followed by the substrate solution (luciferase/d-luciferin).
Briefly, DPPH solution was prepared by diluting 1.2 ml of DPPH stock solution (0.2 M DPPH in ethanol) with 3 ml ethanol and 0.5 ml DMSO.
