Briefly, responder mice carrying either the Tau-transgene together with a luciferase reporter were crossbred with the CaMKIIα-tTA transactivator mice [ 47] to obtain double-transgenic mice with constitutive expression of luciferase and pro-aggregant TauΔK or TauRDΔK.
Briefly, responder cells were aseptically loaded with 0.2 μmol/l CFSE, resuspended in RPMI 10% foetal bovine serum, and seeded (50,000 cells/well) in replicate wells in a standard 96-well culture plate for 5 days in the presence of plate-bound anti-CD3.
Briefly, responders (R) to drug-treatment were defined as showing no change or regression, in TVol, defined as ΔTVol ≤ 10%, while all others were considered non-responders (NR).
Briefly, PBMCs (responder) were prepared from vaccinated chickens as previously described [ 13], and cultured together with stimulator cells at three different ratios.
Briefly, recipient splenocyte responder cells were stimulated for 6 days with irradiated donor or 3rd party splenocytes.
Briefly, we used as responder cells freshly isolated splenocytes stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen) and plated them in the presence of anti-CD3 mAbs (5 μg/ml) in the bottom well of a 96-transwell plate (0.8 × 10 cells/well).
In addition, time to progression and PFS, which can be assessed in all patients, as opposed to duration of response that can only be quantified in responders, were briefly discussed as alternative endpoints in certain circumstances (i.e., investigation of noncytoreductive anticancer agents) [ 7, 8].
Briefly, CD4- or CD8-enriched responder cell populations were used at 4 × 10 cells per well (in duplicate) and peptides were added to a final concentration of 10 μg ml−1.
8 Briefly, a symptom survey asked responders to rate the importance of a range of factors that could influence management choices, and semi-structured telephone interviews with a sample of survey responders explored decision-making processes, identifying other factors influencing management choices.
In Southern California, Trump also met briefly at an airport hangar with families and first responders touched by the shooting at the Borderline Bar & Grill in Thousand Oaks more than a week ago.
Briefly, DC were harvested from D9 BM culture and used as stimulator cells to activate responder (T cells).
