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Briefly, reduction of HED by reduced glutathione was measured in an MIF-catalyzed reaction.
Briefly, reduction was carried out using 5 mM dithiothreitol (DTT, 60°C for 45 minutes) followed by alkylation using 20 mM iodoacetamide (room temperature for 10 min).
Briefly, reduction was carried out by incubating 500 μg of total protein lysate with 5 mM Dithiothreitol (DTT) at 65°C for 45 min and was then alkylated with 20 mM iodoacetamide at room temperature for 10 min to irreversibly modify cysteine.
We were able to enrich for these progenitor cell fractions following the parameters described by Stingl et al. Briefly, reduction mammoplasty tissue obtained from 6 independent patient samples was subjected to a series of enzymatic dissociations to obtain a single cell suspension.
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Briefly, GSSG reduction and NADPH consumption were followed at 340 nm.
Briefly, the reduction potential of the homogenates converted Cu2+ to Cu1+.
Briefly, the reduction of mtDNA during early development 'redistributes' mtDNA to daughter cells (effectively sharing mtDNA content amongst daughter cells).
When the next speaker, turning to possible solutions, referred briefly to a "reduction of salaries," the audience froze, but a second later I could sense some relief.
The environmental benefits of energy losses reduction were briefly presented.
The theory of echo reduction is briefly discussed, the inverse method is developed and then an experiment is conducted to illustrate the technique.
Obama did briefly argue that deficit reduction should be spearheaded by ending Bush's tax cuts and by ending subsidies for unimaginably profitable oil companies.
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