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During the Younger Dryas age, woolly mammoths briefly expanded into north-east Europe, whereafter the mainland populations became extinct.
Colonies were isolated using a pipette tip and briefly expanded in adherent cultures before isolation of genomic DNA using the DNAeasy kit (Qiagen).
Following this first boost, Ad5-specific CD8+ T-cell responses returned to pre-vaccination levels in baseline Ad5 seropositive subjects, and were only briefly expanded again at weeks 30 and 42 following the 2nd boost at week 26 (p<0.04).
Fibroblasts explanted from donors of different ages, then briefly expanded in culture, also showed a moderate reduction in HuR levels as the age of the donor increased [ 49].
After SCA1+CPC transduction of the indicated lentiviral vectors (Supplementary Figure S2a), positively transduced cells were sorted, briefly expanded, and total RNA was isolated using the mirVana kit (Ambion, Madrid, Spain).
Briefly, expanded OC-derived cells and sorted OC cells were released by trypsin treatment, counted and resuspended in 15 ml polypropylene conical tubes at a density of 2 × 10 10, and short spun down at 500 g.
Similar(49)
When Symphony Space was spruced up, the company followed suit, upgrading its productions and casting but offering shorter seasons, and briefly expanding its area of specialization beyond its namesakes' works to musical comedies by the Gershwins.
Relatively small objects like our sun can live up to 10 billion years before briefly expanding into red giants and then slowly dying as white dwarfs.
And so, we see his world briefly expand and as good drama does, we see it briefly expand and we have hope that Benny is going to find some happiness and then we see all these problems arise and we see his world shrink again.
We see [his world] briefly expand and we have hope that Benny is going to find some happiness and then we see all these problems arise and we see his world shrink again..
After briefly expanding the selected cells, we used flow cytometry to further select for ULBP1lowCD55+ cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com