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Briefly, collected serum (10 ml) was combined with 1 ml of color A reagent (including urease) and incubated at 37 °C for 15 min.
Briefly, collected cells were ground with liquid N2 and extracted in 0.2 M phosphate buffer, pH 6.5, containing 0.25% (v/v) Triton X-100 and 3% (w/v) polyvinylpolypyrrolidone (PVPP).
Briefly, collected inflammatory cells were washed and resuspended at 1 × 10 cells in Hank's buffered saline solution.
Briefly, collected media of an appropriate volume were prepared with SDS sample buffer without boiling or reduction, and subjected to 0.1%gelatin-8gelatin-8%electrophoresisis.
Briefly, collected heart tissues were homogenized with modified RIPA buffer, the supernatant centrifuged twice at 4,300 g for 10 min at 4°C then stored at −80°C until further use.
Blood samples were briefly collected and centrifuged (1000 g for 10 minutes at 4°C), and all plasma samples were frozen at −80°C for biochemical and hormonal analysis.
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He added that the 2015 USA Freedom Act, which modified the Patriot Act, provided the FBI with emergency powers to briefly collect online metadata before receiving a judge's approval.
In this section we briefly collect some fixed point results which are consequences of the results presented in the main section.
The remainder of this paper is organized as follows: in Section 2 we will briefly collect some necessary well-known facts; in Section 3 we will give the proofs of the main results in this paper.
Briefly, the collected hemocytes were resuspended in 1 mL of PBS.
Briefly, the collected blood fed females were stored in net covered paper cups or in cages and provided with cotton wool moistened with sucrose.
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