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Briefly, cells were fixed using citrate/acetone fixative solution for 30 s, followed by rinsing twice with deionized water.
Briefly, cells were fixed in Boum's fixative, treated with 5M HCl for 45 minutes then stained with Schiff reagent.
Briefly cells were fixed with fixation buffer for 20 minutes and after washing incubated with permeabilisation buffer for 20 minutes.
Briefly, cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 20 min at RT, and permeabilised in 70% ethanol overnight at 4 °C.
Briefly, cells were fixed in a complete cell fixation solution for 15 min and then washed twice with ice-cold PBS and resuspended in 1 ml chromatin prep buffer.
Briefly, cells were fixed for 10 min with 1% formaldehyde, followed by addition of 0.125 mol/L glycine for 5 min to stop cross-linking.
Briefly, cells were fixed with 1% formaldehyde.
Briefly, cells were fixed for at least 30 min in 95% ethanol.
Briefly, cells were fixed onto chamber slides using 0.1 M phosphate buffered 2.5% glutaraldehyde, pH 7.4 overnight.
Briefly, cells were fixed and stained with anti-E-cadherin, anti-Hic-5 and anti-STAT5b antibodies.
Briefly, cells were fixed in 2% paraformaldehyde in PBS, washed with PBS and permeabilized with 0.2% Triton X-100 (Sigma).
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