Sentence examples for briefly assays from inspiring English sources

Briefly, assays contained potassium phosphate buffer (50 mM for Trx reductase assays, 500 mM for selenocystine reductase assays, and 100 mM for all other substrates), 1 mM EDTA, 150 μM NADPH, and varying concentrations of each substrate.

Briefly, assays were performed on both the reference laboratory strain 3D7, as well as on the multidrug resistant strain Dd2, where parasite growth was evaluated using LDH activity [17].

Briefly, assays were performed by recording the first 2 min of GS-CDNB conjugation at 25 °C in the reaction mixture consisting of 100 µL of 50 mM GSH (Sigma-Aldrich, Taufkirchen, Germany), 40 µL of 50 mM 1-chloro-2,4-dinitrobenzene (CDNB) (Sigma-Aldrich, Taufkirchen, Germany) dissolved in ethanol, 10 µL of enzyme extract and 0.1 M potassium phosphate buffer, pH 6.5, in a total volume of 2 mL [24].

Briefly, assays were performed in with reduced lighting, simply by the addition of 20 µl of CellTiter AQ reagent into the relevant samples in 100 µl of culture medium.

Briefly, assays were performed in 96-well polypropylene microplates(Corning-Costar 3790, NY, USA).

Briefly, assays were performed in a total of 20  μL in 384-well plates using fluorescence resonance energy transfer technology.

We explain our mission to him, how we're looking to find out who might be the best This Is the End guy to hang out with in a calamity and that it's not exactly a serious undertaking, all we're attempting to do is briefly assay the personality in front of us.

Briefly, assay mixtures contained 50 mM Tris HCl (pH 7.2), 10 mM MnCl2, 0.76 μM GDP-[C] mannose (0.01 μCi; specific activity 262 mCi/mmol), 50 mM α-methylmannoside as acceptor, and 200 300 ng protein.

Briefly, assay mixtures were incubated for 45 minutes at 70°C in thin-wall PCR plates before precipitating the radiolabeled transcripts by the addition of ice-cold TCA solution.

Briefly, assay plates were coated with monoclonal anti-human CCN2 IgG1 raised against a peptide from the carboxyl-terminal domain of CCN2 (MAB660, R&D Systems, Abingdon, UK) and incubated at 4°C overnight (20 μg/ml in phosphate buffered saline [PBS]).

Briefly, assay reactions (in triplicate) were conducted at 30 °C measuring the absorbance during a total time of 30 min each 1 min in a Synergy HT Multi-Mode Microplate Reader (BioTek, Bad Friedrichshall, Germany).