Cells were briefly washed out with regular HEPES buffer and continuously perfused with HEPES buffer without RANKL and M-CSF.
Briefly, individual oocytes were placed in the recording chamber and continuously superfused with frog Ringer's solution.
Briefly, slices were placed in an interface chamber (Fine Science Tools) and continuously superfused with oxygenated ACSF (artificial cerebrospinal fluid; 30°C) at 1 3 ml/min.
Briefly, cells were plated in six-well plates (5 × 105 per well) or in culture tissue-flask (15 × 10) and continuously exposed for 4 days to 100 μg/ml of PSK.
The experiment compared direct user interactions during pointing at a target, which was continuously visible or presented briefly and disappeared, in both stereoscopic and real environments, at three parallax/depth levels.
"It's timeless and continuously entertaining," he said.
"They lobbied actively and continuously for one whole year.
Everything is instantaneous and continuously updated.
All this happens quickly and continuously.
Briefly, the lateral cochlear wall with stria vascularis was mounted in a perfusion chamber on the stage of an inverted microscope (Nikon TE-300) and continuously perfused with the same solution used for the micro-Ussing chamber at 37°C.
