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For this design brief, we used the Activity Analysis and Affinity Diagrams listening methods.

In brief, we used the mMESSAGE mMACHINE T7 Ultra kit (ThermoFisher Scientific, #AM1345) to synthesize Cas9 mRNA.

In brief, we used metallic gallium, ammonia (NH3) gas, hydrogen chloride (HCl) gas, and hydrogen (H2) as carrier gases.

In brief, we used a yeast strain that is prone to vesicle production due to an engineered defect in secretory-vesicle/plasma-membrane fusion.

In brief, we used Rhaghavan's label propagation method seeded with node labels determined by Newman's leading eigenvector modularity maximization method to assign cluster membership to each node [27, 28].

In brief, we used a linear array of 4 microelectrodes made of tungsten wires insulated by epoxylite, each spaced 625 µm apart (FHC n°MX41XBWHC1).

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Throughout this brief, we use P T, λ min ( P ) ( λ max ( P ) ) to denote the transpose and the minimum (maximum) eigenvalue of a square matrix P, respectively.

In brief, we use the following approach to perform and present numerical simulations.

In brief, we use a non-cell permeable dye to determine the 3D shape of cells and measure the total amount of fluorescence within the entire cell volume.

In brief, we use GeneSign to identify the transcriptomic fingerprints of murine immune cell types and we subsequently use these home-made gene sets in BubbleMap to assess their enrichments in human immune cell types (see the online User guide for a detailed step-by-step procedure of how to repeat this data analysis workflow).

For a brief time, we used a Salvation Army site, and then we moved to another undisclosed church site that still wasn't big enough.

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