Sentence examples for brief tissue samples were from inspiring English sources

In brief, tissue samples were removed and snap-frozen with liquid nitrogen.

In brief, tissue samples were homogenised in a solution containing 5% trichloroacetic acid and 5 mM EDTA at 4°C.

In brief, tissue samples were embedded in paraffin blocks, cut into 3  μm sections, deparaffinized, and rehydrated.

In brief, tissue samples were placed in 4 M dithiothreitol (DTT -GITC/sarc (4 M guaniDTT -GITC/sarccyanate, 50 mM Tris-HCl, pH 7.5, 25 mM EDTA) (Invitrogen: cat. no. 15577-018).

In brief, tissue samples were embedded in optimum cutting temperature compound (O.C.T. compound, Tissue-Tek, Sakura Finetek Europe, Netherlands), immediately frozen in −78 °C isopentane and stored at −80 °C until use.

In brief, tissue samples were fixed in 4% buffered formalin, embedded in paraffin, 2  μm sections were deparaffinised and antigen-retrieval was done by heating in 0.01  citrate buffer, pH 6.0.

In brief, human tissue samples were fixed in 10% neutral-buffered formalin and paraffin-embedded.

In brief, frozen tissue samples were individually thawed, weighed, and homogenized in PBS.

In brief, harvested tissue samples were lysed with proteinase K (>600 mAU/ml).

In brief, fresh tissue samples were rinsed, cut into about 2 mm pieces, and then transferred to 60 mm culture dishes.

In brief, synovial tissue samples were separated from rat joints and were first homogenised in a solution containing 20 mM potassium phosphate buffer, pH 7.0, to 1 : 10 (w/v) and then centrifuged for 30 min at 20,000 g and 4°C.