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In brief, the amount of protein was determined using the Dc Protein Assay Kit (Bio-Rad, Hercules, CA, USA) following the manufacturer's instructions.
In brief, the amount of starting total RNA for each probe preparation varied between 2 and 5 μg.
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In brief, the amounts of organic and inorganic tin species impurities in the organotin chloride reagents were estimated by GC ICP MS.
In brief, the starting amount of total RNA for each probe preparation varied between 2 to 5 μg.
His organization's brief emphasized the amount of information currently available from interconnected databases.
For subscriber are twitter all over the world the messages show up as microblogs which are brief in the amount of text posted in comparison with regular blocks.
In brief, the input amount of RNA for the DASL assays was 400 ng of total RNA obtained from FFPE blocks.
In brief, the equal amount (0.4 μg) of protein from each sample was incubated with a biotinylated telomerase substrate oligonucleotide (P1-TS primer) at 25°C for 20 minutes.
In brief, the indicated amounts of Abs were added to the samples (10 to 20 μl), mixed and incubated for 5-30 min on ice before loading them on BN-PAGE.
In brief, the relevant amounts of docetaxel or EL102, or combinations of both were preloaded into a 96-well plate (Sarstedt, Ireland) using the Perkin Elmer Janus Automated Workstation.
In brief, the equimolar amounts of the corresponding oligonucleotides were mixed in hybridizing buffer (50 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2), heated at 85 °C for 3 min and cooled slowly to room temperature to anneal.
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