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In brief, testes were fixed in Bouin's and prepared for histology.
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For H&E histological staining, testes were fixed by immersion in Bouin's fixative and embedded in paraffin.
Embryonic testes were fixed for 1 hr in Bouin's fixative (3 hr for PND4 testes) then transferred to 70% ethanol.
Testes were fixed for 6 hours in Bouin's fixative, dehydrated in a graded ethanol series, and embedded in paraffin.
Testes were fixed for 6 hr in Bouin's fixative and then transferred to 70% ethanol until analysis.
After collection, testes were fixed overnight (o/n) in 4% PFA or Bouins fixative and embedded in paraffin using standard procedures the following day.
The testes were fixed in Bouin's fluid and embedded in paraffin wax after dehydration in ascending series of ethyl alcohol.
For IHC, testes were fixed in formalin.
Testes were fixed, embedded, and sectioned as described above.
Testes were fixed by immersion in 4% paraformaldehyde in PBS at 4°C for 40 h.
Five days after the microinjection, testes were fixed with periodate-lysin-paraformaldehyde (PLP).
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