In brief, rat tail collagen (2.2 mg/ml, First Link, Birmingham, UK) was mixed with 10× DMEM Life Technologiess, Paisley, UK) at a ratio of 9 1 and the pH was adjusted to approximately 7 by the addition of 10 M NaOH.
In brief: the tail skin of C57BL/10 mice was removed and placed onto the back of anesthetized (mixture of ketamine and xylazine) BALB/c recipients and fixed by a splint around the thorax.
In brief, mouse tails were incubated overnight in 100 mM Tris, pH 8.0, 0.5 mM EDTA, 200 mM NaCl, 0.2% SDS, and 100 µg of proteinase K at 55°C.
In brief, the tails were sprayed with a 20% tincture of benzoin (Styrax spp.; Rioquimica, Rio de Janeiro, RJ, Brazil).
In this respect, it is interesting to note that the tail-flick is classically described as a brief movement of the tail, with the reaction time being shorter [20] [22], [24] [27] and the movement more vigorous [21], [28] when the intensity of the source of radiant heat increases.
The two dogs lay on the front porch, lifting their muzzles at the sight of the familiar car, some brief motion from each tail, but neither bothering to leave his spot.
The sight of Victoria Azarenka collapsed in a heat-ravaged heap, then carried from the court in a wheelchair on her way to hospital, underlined the physical demands of this US Open, with only brief relief from the tail feathers of Hurricane Earl in sight tomorrow and 11 days to go in New York's hottest ever summer.
They had a brief run at the tail end of last year, to qualify for Oscar nominations; now, at their leisure, they are enjoying a wider release, and, for that full revolutionary flavor, I would recommend seeing them back to back, with a pit stop for a mojito in between.
When they departed either side of Neil Dexter falling to Jack Brooks (4-33), a brief wag of the tail ensued before the seamer claimed the final three wickets.
