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In brief, specimens were deparaffinized and immersed into 10% MgCl2 solution for 4 hours.
In brief, specimens were scored as pAKT positive if >20%or=20%% of the tumour cells demonstrated staining at an intensity equal to or greater than that of the positive control.
In brief, specimens were 1 1 mixed with N-acetyl l-cysteine (NALC -NaOH (fiNALC -NaOHtrations 1.5% NaOH, 0.7% NaCitrate, 0.25% N-acetyl-cysteine) and put on plat finalshaker (Thermo sconcentrations USA) at 60 rpm for 20 min.
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In brief, the specimens including the trephines were fixed in neutral-buffered formalin, stored in compartment biopsy cassettes, and appropriately coded for identification.
In brief, blood specimens were frozen and stored at −70°C until analysis.
In brief, stored specimens were returned to room temperature just before testing.
In brief, all specimens were fixed in 4% formalin for 24 hours, then dehydrated and embedded in paraffin.
In brief, brain specimens of ~100 μg were solubilized in 300 μl RIPA buffer before isolating the protein fraction, as described in our earlier report [ 16].
In brief, the specimens were incubated with the primary antibody, and then reacted with species-specific biotinylated secondary antibodies, followed by streptavidin-HRP and DAB.
In brief, myocardial specimens were cut into fragments less than 1 mm and cultured as cardiac explants on fibronectin-coated dishes.
In brief, surgical specimens from the neocortex and hippocampus were fixed in formalin and sectioned perpendicularly to the cortical surface into several tissue blocks.
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