In brief, genomic DNA extracted from sorted WT or Jnk-deficient GFP+ cells was digested with EcoRI, which cuts once in the provirus.
In brief, cell suspensions of 1,000 sorted mouse luminal mammary cells were seeded in Mouse EpiCult-B (StemCell Technologies) and 50 μg/ml gentamicin in the presence of irradiated feeders for 6 to 7 days.
Sorted cells were expanded and the brief bicalutamide treatment and FACS-sorting was repeated (four rounds).
In brief, seven pools of flow-sorted whole chromosome painting probes were amplified and directly labeled using seven different fluorochromes (DEAC, FITC, Cy3, Cy3.5, Cy5, Cy5.5, and Cy7) using degenerative oligonucleotide-primed PCR (DOP-PCR).
In brief, BAM files were binary compressed, sorted, and indexed by SAMtools (samtools view, sort, and index tools), duplicated reads were removed by Picard (with MarkDuplicates), and base quality score recalibration and local realignment around indels followed the recommended workflow of the GATK toolkit (RealignerTargetCreator, IndelRealigner, BaseRecalibrator and PrintReads).
Sorted cells were expanded in culture (without drug) until they reached approximately 60 million cells, we then isolated gDNA and froze down a small fraction, and the brief bicalutamide treatment and sorting was repeated on the remainder.
Sorted cells were expanded in culture (without drug) until they reached approximately 60 million cells, we then isolated gDNA and froze down a small fraction, and the brief enzalutamide treatment and sorting was repeated on the remainder.
In brief, NSCLC cells at exponential growth were treated with various reagents or FACS-sorted.
