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In brief, sections were mounted on gelatin-coated slides, incubated for 1 hour in 5% normal donkey serum and 0.3% Triton X100 in PBS and then overnight at room temperature with the primary antibody solution containing 0.3% Triton X100 and 2% normal donkey serum.
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In brief, 90nm sections were mounted on 200 mesh copper grids before double staining with uranyl acetate and lead citrate.
In brief, 5 μm paraffin sections were mounted on poly-L-lysine coated slides for heat-induced epitope retrieval ('HIER' technique) in citrate buffer.
Following brief fixing (8% formalin in PBS for 20 min) and washing (PBS+10 mM EDTA), AP-h-S100A7 stained tissue sections were mounted with aqueous mounting medium and stored in the dark at room temperature.
After staining, sections were mounted in DPX.
Sections were mounted with ProLong Gold Antifade Reagent (Life Technologies, Inc.).
Sections were mounted on #1.5 glass coverslips using PVA-DABCO mounting media.
Sections were mounted with DPX.
Sections were mounted in mowiol for imaging.
Sections were mounted with Vectashield containing DAPI.
Sections were mounted using Hydromount (National Diagnostics).
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