In brief, antigen retrieval was performed in target retrieval solution (Dako, Carpinteria, CA, USA) using a Decloaking Chamber Biocare Medicall, Concord, CA, USA).
In brief, antigen retrieval was performed using heat treatment with Target Retrieval Solution (pH 9.0) (S2367, Dako, Glostrup, Denmark) at 95 99 °C for 20 min.
In brief, antigen retrieval at 98°C for 30 min in Target Retrieval Solution (DakoCytomation) was followed by incubation with the affinity-purified LYVE-1 Ig (0.5 μg ml−1) for 30 min.
In brief, antigen retrieval was performed by incubating the sections in 0.1 M citrate buffer, pH 6.0, for 15 min. Endogenous peroxidase was blocked by incubation with 3% H2O2 in 100% methanol for 5 min. Nonspecific epitopes were blocked with a mixture of 0.1 M Tris-buffer, pH 7.5, 3% bovine serum albumin and 20% normal bovine serum for 1 h.
In brief, antigen retrieval was performed in a microwave oven in 0.03 M sodium citrate acid buffer.
In brief, antigen retrieval was performed by placing the unstained slides in 0.01 mM citrate buffer (PH 6.0) and treating them in a microwave oven for 10 min. Overnight incubation at 4°C was performed for anti -pan-Trk, -p75NTR, -pc-Jun and -pJNK primary antibodies and 1 hour at room temperature incubation was performed for anti-TrkA, -TrkB and -TrkC primary antibodies.
In brief, antigen retrieval was performed in 10 mM citrate buffer (pH 6.0) for 20 min with wet heat (hot bath) at 98°C for PDGFR-α or, for PDGF-A, samples were pretreated in 10 mM citrate buffer for 15 min (3 x 5 min) in a microwave at 600 W. After cooling to room temperature, the sections were rinsed with PBS, which was used for all subsequent washing steps.
In brief, the antigen retrieval was performed by heating the sample in 0.01 M citric buffer (pH 6.0) 2 × 5 min in a microwave at 750 watt.
Modern information retrieval: A brief overview.
