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No accessorizing with survival gear except for a brief proliferation of utilitarian sneakers and backpacks - quick getaway chic, if you will.
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We wished to determine methods that could reveal how the cell cycle is regulated in non-immortalised, very early MEC cultures, and therefore examined ways of extending this brief window of proliferation that characterised the first 3 5 days of primary cell culture, using cells from pregnant mammary glands.
In brief, morphology and proliferation of cells were routinely assessed and the identities of cells were verified by isoenzyme and short tandem repeat analysis.
Consistent with previous observations (Michaloglou et al, 2005), we show that BRafV600E expression caused growth arrest of both normal human cells and hTERT-expressing cells after a brief period of proliferation (Supplementary Figure S10A and B).
The level of initiation by DMN can be further enhanced by unilateral nephrectomy or hydronephrosis, which induces a brief burst of cell proliferation followed by tumorigenesis in the contralateral kidney.
As someone who came of age during the era of South America's dirty wars, it is understandable that he should side with the view he attributes to one of the characters in 2666, who sees history as a "simple whore... a proliferation of instants, brief interludes that vie with one another in monstrousness".
Our examination of BrdU incorporation thus provides only a snap shot of proliferation over a brief time period and not a quantitative measure of the total increase in proliferation.
In brief, hemocyte clusters enhance hemocyte proliferation and provide structure as to guarantee the necessary cell contacts that engage the signaling events behind cell fate decisions.
The majority of neurons in the adult neocortex are produced embryonically during a brief but intense period of neuronal proliferation.
Expression of PKC can be induced in all stages of the time proliferation curve after a brief pretreatment with phorbol esters such as PMA.
In conclusion, the only test to show potential as a predictive measure of pathogenicity was that of cell proliferation in lungs after brief inhalation exposure (BrdU assay).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com