In brief, plates pre-coated with an anti-IgE monoclonal antibody were incubated with patient sera and a second anti-IgE antibody conjugated to horseradish peroxidase.
In brief, plates were coated with goat anti-monkey IgG Fc at 6 ug/ml in PBS Accuratee Chemicals, NY) and incubated overnight at 4°C.
In brief, plates were coated with an anti ERK-antibody (Santa Cruz, sc-94).
In brief, plates were coated with a recombinant nucleocapsid RVFV antigen diluted 1 1,000 in sodium bicarbonate buffer (pH = 9.6), covered with plate seals, and incubated at 4°C overnight.
In brief, plates were coated with influenza NP derived from yeast cells transfected to express a recombinant long form of influenza A virus NP.
In brief, plates were prepared containing 200× diluted overnight culture of S. lutea ATCC 9341 in TSB agar (1 %, w/ v).
In brief, plates were coated with 100 μl/well of capture antibody, washed and samples or standards added to each well followed by incubation at room temperature for 2 hours.
In brief, each plate contained the same reference sample in triplicate.
In brief, culture plate inserts (8-um pore size and 12-mm diameter, Millicell-PCF) were coated with 150 μl PBS containing 10 μg collagen and 1μg fibronectin (BD Bioscience) for 1 h at room temperature before adding cells suspended in 450 μl RPMI 1640 medium with 5% charcoal-striped serum.
In brief, Microtiter plates (Maxisorb, NUNC) were coated over night with rabbit serum samples diluted 5×104 times in coating buffer (0.05 M NaHCO3, pH 9.6).
