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In brief, mice were randomized, anesthetized, and underwent a partial laminectomy at thoracic level T8.
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In brief, adult (8-week old) male C57BL/6 J mice were randomized to receive high-fat diet (HFD, 60%% kcal, Research Diets Inc. D12492i) or normal diet (ND, 10%% kcal control, D12450Bi) containing the same protein content as HFD.
Tumor-bearing mice were randomized into treatment groups, when necessary.
A total of 12 mice were randomized into 4 different groups.
When the volume of xenografts reached approximately 100 mice mice were randomized into groups and treatments initiated.
The remaining 42 mice were randomized to receive vehicle injection (14 mice), SC-NES cell injection (19 mice) or NCX-NES cell injection (9 mice) at 10 days after hemisection.
Once tumors grew to a palpable size (~ 500 mice, mice were randomized and treated daily with 20 mg/kg (body weight) PLX4720 via intraperitoneal injection.
Upon establishment of tumors (150 250 mice, mice were randomized into two groups: vehicle (0.5% methylcellulose, 4% HPMC-AS) or NB-598 (300 mg kg−1).
Sixty female inbred BALB/cJ mice were randomized into six tibial nerve transplant groups, including three isograft and three allograft (C57BL/6J) groups.
Three days later after inoculation or after tumour growth up to 5 × 5 mm or 7×× 7 mm, mice were randomized into different groups (sample size was n=5 10) based on similar tumour size and body weight.
After 3 4 days post-surgery recovery the WT and TG mice were randomized to the following 48 h i.v. infusions (1) SAL (WTSAL; TGSAL) or (2) OLE (WTOLE; TGOLE) to elevate plasma NEFA by 1.5 2 fold.
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