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In brief, mice were placed on a 55°C hotplate and observed by two individuals masked to treatment.
In brief, mice were placed in a 10 cm wide glass cylinder on a hot plate (Hot Plate HP-2002, Insight Equipamentos, Ribeirao Preto, SP, Brazil) maintained at 55°C.
In brief, mice were placed in transparent acrylic enclosures (7 × 9 × 11 cm) with a glass plate and allowed to acclimatize to their environment for 1 h before testing in a temperature-controlled and noise-free room (23 ± 2°C).
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In brief, the mice were placed on an operating table at 37.5 °C under a dissecting microscope.
In brief, for elevated plus maze, mice were placed in a cross-shaped maze 65 cm above the floor with 2 open arms (20 × 8 cm), 2 closed arms (20 × 8 × 25 cm) and a central area (8 × 8 cm) in uniform dim lighting.
5 In brief, for the rota rod test, mice were placed on a rod rotating at 30 rpm, and the time taken for them to fall from the rod was measured.
In brief, after an intraperitoneal injection of 1% ketamine (30 mg/kg) and xylazine hydrochloride (4 mg/kg), the mice were placed in a stereotaxic frame (Kopf Instruments, Tujunga, CA).
The mice were placed in the prone position.
Infected mice were placed in isolators.
Unanesthetized mice were placed in plastic holders.
Control mice were placed in restrainers only.
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