In brief, labeled EVs (70 μL in TBS with 0.05% Tween-20 (TBS-T)) were loaded on to each well of the gasket slide at a concentration range of 0.75 μg/mL for lectin microarrays, 50 or 25 ng/mL for glycan microarrays and 250 ng/mL for mammalian lectin microarrays based on EV protein concentration.
The arrows between the pedagogical components communicate directionality of relationships among pedagogical components and have brief labels describing the relationships.
In brief, labeled RNA was dissolved in 16 ul hybridization mixture (15% formamide; 0.2% SDS; 3×SSC; 50×Denhardt's) and hybridized overnight.
In brief, labeled cells were fixed with 4% paraformaldehyde and images obtained with a Leica DM IRE2 microscope [66], [67].
Direct observation of endocytosis following brief labeling with FM4-64 indicathatthat endocytosis occurs preferentially at the rhizoid pole.
In brief, labeling of 1 μg DNA was performed using the BioPrime Total Genomic Labeling System (Invitrogen Corporation, Carlsbad, CA) following the manufacturer's protocol.
In brief, labeled antibodies were detected with the ZeptX system, in which liquid from each well is pumped through an interrogation space within a capillary flow cell.
In brief, RNA labelling and amplification was performed with Low Input Quick Amp Labeling Kits, Two-Color and RNA Spike-In KiTwo-Colorolor for 4 x 44 K microarrays, using 200 ng of total RNA per reaction.
In some galleries iPads are mounted to allow more extensive explanations than the brief informative labels.
This was Melvins' brief major-label period – they had signed to Atlantic after the grunge explosion, when similarly unconventional groups such as Jesus Lizard, TAD and Butthole Surfers were scooped up by A&R folk hungry to capitalise on Nirvana's success.
