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This laser vaccine adjuvant (LVA) effect was induced by brief illumination of a small area of the skin or muscle with a nondestructive, 532 nm green laser prior to intradermal (i.d).
This investigation provides proof of concept evidence that brief illumination of skin or muscle with non-destructive, safe, green laser can prime the body to a stronger response to a protein prototype vaccine that, by itself, induces relatively weak immune responses.
Importantly, brief illumination of the cells with UV light led to rapid entry of OptoNLS-FOXO3-mCherry into the nucleus.
GnRH neurons were identified by brief illumination 15-455 sec) at 470 nm to visualize the GFP signal.
In our next line of investigation, we examined the re-accumulation of protochlorophyllide in the dark after a brief illumination as a means to analyze the kinetics of protochlorophyllide synthesis in the seedlings.
All images were focused during brief illumination with a laser beam and collected with a single scan using a low laser power to avoid photooxidation of CM-H2DCFDA, which has been reported previously (Chen et al. 2005).
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Each session consisted of 100 completed trials and lasted a maximum of 30 min. Rats were trained to make nosepoke responses into the aperture in the front array upon brief stimulus illumination of the light located therein.
While both brief light pulses as well as prolonged illumination increase carotene accumulation in Phycomyces (Bejarano et al. 1991), continuous illumination results in even decreased carotene production in Blakeslea (Sutter 1970).
After the stimulation is exhausted, the crystal can be re-activated by a brief ultraviolet illumination, and the process of obtaining multiple flashes by tapping can be repeated.
Compact components are under development for a 3-D active imaging system in which the vertical and horizontal dimensions are augmented with the range dimension as obtained by timing the return of photons from a brief laser illumination pulse.
Brief laser illumination may transiently alter the interstitial microarchitecture and increase the permeability and flow of interstitial macromolecules or cells to lymphatic capillaries as a result of photothermal and photomechanical effects.
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