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This brief freezing firms up the pastry cream so that it cuts cleanly.
Airway bleeding and mucosal tears may have been avoided due to brief freezing times (no longer than 3 seconds) limiting the depth and extension of tissue adherence to the cryoprobe.
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In brief, frozen eight-micron sectionsections from OCT-preserved frozen tissues were prepared and mounted on plain, uncharged microscope slides.
In brief, frozen carcasses were weighed (W1) and autoclaved in individual sealed beakers with distilled water (20% weight of the carcasses) for 2.5 h at 120°C.
In brief, frozen human cortical samples were physically homogenized and powdered.
In brief, frozen samples of lung were thawed and homogenized in ice-cold buffer provided in the kit.
16 In brief, frozen urine specimens were brought to room temperature and centrifuged at 10 000 g for at least 5 min prior to testing.
In brief, frozen sections or cells were washed three times with PBS and fixed for 30 min at RT with fixative solution.
16 In brief, frozen 100 mg sections of the DLPFC sent from the Rush Alzheimer's Disease Center were processed at the Broad Institute in Cambridge, MA.
In brief, freeze dried tissue samples were extracted with methanol:water (2 1 v/v) by homogenization (Tissue Lyser, Qiagen, Hilden, Germany), followed by sonification for 20 min and incubation at 50°C for 30 min in a shaker.
In brief, frozen sections 5 (5 m thick) were prepared, transferred onto poly- L-lysine-coated slides, air-dried, and then washed in PBS, followed by quenching of endogeneous peroxidase activity with 0.3% hydrogen peroxide in methanol.
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