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In brief, for one liter culture of HEK293F cells, 1 mg plasmids was pre-incubated with 4 mg 25-kDa linear polyethylenimine (PEI) (Polysciences) in 50 mL fresh medium for 30 min prior to adding the mixture to cells.
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Google Helpouts, which launched this week, is a service that allows you to pay for brief one-on-one webcam masterclasses with a range of experts in various fields.
He stopped for a brief one-on-one interview with Fox News' Carl Cameron – or as one-on-one as you can get with a few hundred people packed in within a few feet of you.
He was accompanied to the Pentagon by Henry A. Kissing er, his special assistant, and was briefed for one hour and 40 minutes by Secretary of De fense Melvin R. Laird and high miiltary officials in the pres ence of the Joint Chiefs of Staff.
On Sunday's Meet the Press, host David Gregory welcomed talking bumper sticker and executive vice president of the National Rifle Association Wayne LaPierre for a brief one-on-one softball interview.
Although a detailed biological analysis is out of the scope of this paper, we provide a brief analysis for one bicluster per dataset.
Training interventions included both in-person and distance modalities, and ranged from brief (for example, one hour) to extended (for example, one year) training encounters.
She is briefed that, for one of the TV debates, she may be teamed up with Labour's Angela Eagle.
Matchbox Theatre, charmingly packaged by Faber in a slipcase designed to look like a matchbox, consists of 30 brief scenes, usually for one or two speakers.
By brief eosin staining for one second, red blood cells could be stained as red, distinct from the brown signal of DAB.
But for one brief moment on Tuesday night at 8, harsh words were put aside.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com