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Still, at Unnameable Books in Prospect Heights, in the brief fold between night and morning, on a Monday, both books arrived for early-bird purchase.
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In brief 6x10-fold serial dilution was performed on the viral samples followed by 1 hr binding at 37°C on confluent MDCK cells in 6-well plate format.
In brief 6×10-fold serial dilution was performed on the lung homogenate samples followed by 1 hr binding at 37°C on confluent MDCK cells in 6-well plate format.
In brief, 2-fold serial dilutions of serum were incubated overnight with safranin-stained, formalin-killed F. tularensis cells at 35°C, and a titer was assigned according to the last well demonstrating full agglutination.
The TCID50 (50% infectious dose in tissue culture) of the virus was estimated using a previously described method., In brief, 10-fold serial dilutions of allantoic fluid containing the virus were inoculated into Madin-Darby canine kidney cells (MDCK ATCCC No. CCL-34) in a 96-well culture plate and incubated for 3 d at 37°C in a 5% CO2 humidified atmosphere.
In brief, a four fold increase of antibody concentrations between t0 and t3 was considered significant.
Unfold the sides of the brief and then fold it again length-wise.
Usually related to breaking economic and political news, these spots are brief and Reich folds them into his routine without undue strain.
In brief, a two-fold dilution in isotonic NaCl of serum obtained from immunized rabbits was mixed together with 6 × 109 CFU/ml serotype 3 S. pneumoniae (1 1).
In brief, serial 2-fold dilutions of heat-inactivated patient plasma samples were made, from 1 100 to 1 1,600, and were mixed with equal volume of ≈50 100 focus-forming units per milliliter SNV (isolate SN77734, titer 2 × 10/mL) or Andes virus (Chilean strain of human origin, isolate CHI-7913) and incubated at 37° for 1 hour (15).
Three independent assay runs were performed for each sera pool per virus by a single technician using the method detailed below: In brief, serial 10-fold dilutions of the serum pools were mixed with a constant challenge dose of each respective JEV (45 60 plaques per well) and inoculated in duplicate, onto wells of a 6-well plate of confluent LLC-MK2 cells.
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