In brief, fixed cells with 1% formaldehyde were lysed in SDS-lysis buffer (1%SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.1) and sonicated.
Following brief fixing (8% formalin in PBS for 20 min) and washing (PBS+10 mM EDTA), AP-h-S100A7 stained tissue sections were mounted with aqueous mounting medium and stored in the dark at room temperature.
In brief, fixed effects were included for age, sex, cytokine-plate lot, and random effects were included for subject and individual cytokine plate used.
In brief, fixed fetuses were wrapped with a loose thin sponge and immersed in 1×PBS in a plastic container then scanned on volumetric microCT scanner at 27 µm isometric voxel resolution using an eXplore Locus RS Small Animal MicroCT Scanner GE Healthcaree, London, ON, Canada).
In brief, fixed nuclear fractions were suspended in antibody buffer (0.1 M PBS, pH 7.4, containing 0.1% Triton X-100) and incubated overnight at 4°C with mouse monoclonal anti-p53 (DO-1) and rat monoclonal anti-Hsp90α antibody at 50 1 dilution.
Major findings regarding the elements of briefing is that the client should determine the time at which the brief becomes fixed and secondly the brief should be fixed before detail design commences.
After a brief centrifugation, fixed cells were resuspended in PBS containing propidium iodide (5 μg/ml; Sigma)/RNase A (30 μg/ml, Sigma).
In brief, the fixed, paraffin-embedded sections were deparaffinized and washed with PBS, labeled with terminal-deoxynucleotidyl transferase enzyme at 37°C for 0.5 hour, and then incubated with 5-bromo-4-chloro-3-indolyl-phosphate substrate labeled with Cy-3 for 40 minutes and DAPI for 5 minutes at room temperature.
In brief, cultures were fixed with glutaraldehyde and paraformaldehyde, post-fixed with osmium tetroxide, stained en bloc with uranyl acetate, ethanol dehydrated and Epon embedded.
A good way to do it is to use a ticking stop watch - or any kind of device with a bell, timer, beeper, or ring tone - that you can set for a fixed, brief period of time.
