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In brief, extracted viral RNAs were applied to perform the RT-PCR with the primer pair, 10-36F (5′-CTGTGTGAACTTCTTGGCTTAGTATCG-3′) and 85′-CAGTTTTCATGAGATATCGTGTGTGGC-3′TGTGGC-3′).
In brief, extracted nucleic acids and the primers HBV-22, HBV-65, and HBV-66 were used to amplify a 408 bp region of the preS/S-region of the HBV genome.
In brief, extracted genomic DNA (15 ng) was subjected to a 25 µl PCR reaction (SyBR Green) using forward (5′-CATGGTGATGCGGTTTTG-3′) and reverse (5′-CCTCACGACCAACTTCTG-3′) primers amplifying a 333 bp sequence at the 3′-end of the CMV promoter and the vector backbone, between the promoter and the downstream transgene sequence (annealing temperature, 61°C).
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But here's a brief extract Naharin creates striking stage imagery, strongly evoking interpersonal, political and social situations.
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In his book Extreme Fear: The Science of Your Mind in Danger, the science journalist Jeff Wise goes deep into this, but one brief extract (prefacing the tale of a man who did lift a car to save a cyclist trapped underneath) is too relevant to not reproduce here.
Nina Stemme, the Swedish soprano who triumphed in the first Operalia in 1993, gave us Wagner: Sieglinde to Domingo's Siegmund in a brief extract from Die Walküre, and showing in 'Dich, teure halle' from Tannhäuser how far her voice has travelled.
Firstly, the exchange structures appear little different from the universal pedagogic dK1^K2^K1 pattern, but participation differs from common practice in that the teacher usually addresses individual students by name, so that nine students actively participate in turn in this brief extract from the lesson.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com