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In brief, embryos were fixed in 2% glutaldehyde/2% paraformaldehyde for 15 60 min depending on the age of embryo, stained overnight with X-gal solution, and post-fixed with 4% PFA.
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In brief, oocytes and embryos were fixed in 4% PFA (Nacalai Tesque) in phosphate-buffered saline (PBS) for 30 minutes at room temperature, and the permeated samples were then incubated in PBS containing 0.1 0.2% Triton X-100 (Nacalai Tesque) overnight at 4°C.
Embryos were fixed at E4.5 for EPI detection.
Then the embryos were fixed with 1% PFA in PBS in 4°C overnight.
Embryos were fixed using 4% paraformaldehyde in PBS.
Embryos were fixed and processed according to standard protocols [32].
Ae. aegypti embryos were fixed as described [20].
Embryos were fixed overnight in 4% buffered formalin.
The embryos were fixed with 4%PFA at 4°C overnight.
Embryos were fixed and stained as previously described [31].
E14.5 wild-type embryos were fixed with 4% paraformaldehyde overnight.
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