Exact(3)
Our results support the validity of the definition of burnout proposed in the BSCQ-12 through the brief differentiation of clinical subtypes.
In brief, differentiation of U937 cells (5×10 cells/well) was induced in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% FBS, 100 μg/ml of gentamicin and 10 nM phorbol 12-myristate 13-acetate (Merck) for 18 hours.
For detection and counting of amyloid aggregates, spinal cord sections (four to five animals per age/genotype/condition group) were stained in 0.5% Congo red solution in 50% ethanol for 7 min followed by a brief differentiation in potassium hydroxide (0.2% in 80% ethanol) and, after several washes in dH2O, were mounted using Immu-mount (Thermo Electron Corporation).
Similar(55)
The nitrogen isotopic values were also used to describe the chronology of the formation of individual amino acids, as lower δ15N values of α-methyl amino acids, for example, indicate brief isotopic differentiation, and thus an earlier origin in the interstellar medium as opposed to α-H amino acids (Pizzarello and Holmes 2009).
In brief, 14 days after differentiation, primary human adipocytes were treated with 0.5, 1 and 2% BMJ for 24 and 48 h.
To evaluate the differentiation, in brief, cells were fixed with buffered formalin (10%) in PBS for 10 mins at room temperature and stained with oil red-O for 1 h to assess lipid formation for adipogenesis or naphthol-AS-BI phosphate and fast red for 1 h to assess alkaline phosphatase activity for osteogenesis.
This technique enabled (1) the demonstration that oligodendrocytes initiate new myelin segments only during a brief window early in their differentiation, (2) identification of a contribution of astrocytes to the rate of myelin wrapping, and (3) molecular dissection of the role of oligodendrocyte γ-secretase activity in controlling the ensheathment of axons.
Before initiating differentiation, hESCs were given two brief washes in PBS (Invitrogen).
Conversely, a very brief induction of Notch pathway drives the differentiation of post-mitotic cells into mature goblet cells via Hes5 expression [20].
In brief, 2.5 × 10cells/well were seeded in differentiation media with 10% FCS.
Although, it is well known that brief exposure to antigen leads to expansion and differentiation of CD8 T cells, their work proves that full memory differentiation of CD8 T cells remains diminished when the infectious period is shortened.
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