In brief, for detection of surface expressed receptors, cells were incubated with primary antibody (M1 anti-FLAG (Sigma-Aldrich), diluted 1∶1000) for 1h at 4°C.
In brief, EBOV detection was 32.7% (18/55) for carcasses (animals found dead) and 0.2% (13/5309) for live captured animals.
In brief, for detection of sAPPα and sAPPβ, membranes were probed using antibody 6E10 or an anti-sAPPβ antibody, respectively, followed by a S-labeled or peroxidase-coupled anti-mouse or anti-rabbit antibody.
In brief, the detection of CTC appeared to be less sensitive and less prognostic and the prognosis of women with primary breast cancer depended on DTC rather than CTC.
In the study by Clos et al. (2015), however, patients were exposed to lithium for an average of 55 months, possibly too brief to support detection of effects on kidney function (Davis et al. 2015; Bocchetta et al. 2016).
In brief, to facilitate detection of homologous sequences, we translated the cDNA sequence of AK089719 into protein using ReBaseTools (http://rebase.neb.com/rebase/) and then used its deduced amino acid sequence and that of XP_675578 and tblastn 2.2.21 and 2.2.25+ against htgs, nr, est, and est_others with the low complexity filter turned off.
In brief, the variant detection pipeline consisted of the mapping of Illumina reads to the S. pombe genome using BWA, removal of duplicates, indel-based realignment using GATK, base quality score recalibration, mutation calling for a single-nucleotide variant using a GATK Unified Genotyper, and annotation using SnpEff (Sloan Kettering Bioinformatics Core).
In brief, the simultaneous detection of the T cell activation markers CD25 and CD95 was performed by stimulating heparinized whole blood samples with 4.5 μg/mL phytohemagglutinin-L (PHA-L) for 48 h at 37°C, 5% CO2, and 95% relative humidity.
After brief introduction of curvature detection principle of FBG sensor, the design, fabrication and encapsulation of the FBG modular detection unit were illustrated detailedly.
After a brief reminder on the detection principle in Liquid Xenon, the individual detectors will be described.
