Sentence examples for brief cells with from inspiring English sources

In brief, cells with or without ME2 knockdown were incubated with 5 µM MitoSOX™ reagent for 10 minutes at 37°C, then washed three times and observed under a fluorescence microscope using the Rhodamine filter and Axiovision software for capturing images (Zeiss, Germany).

In brief, cells treated with ETME, ATO or the combination for 48 h were incubated with AO (10 μg/mL) and EB (10 μg/mL) for 30 min in dark and then analyzed using HCS.

In brief, cells were washed with PBS, incubated with 10% trichloric acetic acid for 1 h at a temperature of −4°C and washed again.

In brief, cells were fixed with ethanol and stained with 5% silver nitrate for 1 hr.

In brief, cells were incubated with 25 µg/ml BrdU chased with thymidine.

25 In brief, cells were treated with different concentrations of selenite with or without carmustine for 2 hours.

In brief, cells were fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton X-100 for 1 h at room temperature, and then washed twice with PBS.

In brief, cells were treated with ouabain for 24 h in DMEM with 10% FBS.

In brief, cells were washed with PBS (3 × 5 min), fixed with methanol for 10 minutes at −20°C.

ChIP assay was performed as we described, In brief, cells were washed with PBS and incubated for 10 min with 1% formaldehyde at room temperature.

In brief, cells were fixed with 2.5% glutaraldehyde and then postfixed with 1% osmium tetraoxide, dehydrated in a graded series of ethanol concentrations, and embedded in Embed812 resin.