Sentence examples for brief cells were washed from inspiring English sources

In brief, cells were washed and then scraped in cold PBS supplemented with phosphatase inhibitors.

In brief, cells were washed and incubated for 45 min at 37°C with MEM containing 0.5% glucose.

In brief, cells were washed once in wash buffer (Dulbecco's phosphate-buffered saline (DPBS) containing 0.5% BSA without calcium and magnesium).

In brief, cells were washed in PBS and then stained with CFSE at a final concentration of 2.5 µM in PBS with 0.1% FCS (Fetal calf serum; PAA) for 10 min at 37°C.

In brief, cells were washed with KHB and incubated at 37 °C for 30 min.

In brief, cells were washed in PBS and resuspended in 1% (w/v) paraformaldehyde in PBS.

In brief, 1×106 cells were washed once in 1×PBS and were stained with Annexin V-FITC and PI (2 mg/mL) according to manufacturer's instructions.

In brief, CCE cells were washed and lysed in ice-cold RIPA buffer (Upstate, Lake Placid, NY) containing a protease inhibitor cocktail (Roche Diagnostics).

In brief, the cells were washed, collected in ice-cold PBS in the presence of phosphate inhibitors and centrifuged at 500×g for 5 min. The resultant pellets were re-suspended in excess hypotonic buffer, treated with detergent and centrifuged briefly at 14,000×g for 30 sec.

In brief, S11E cells were washed twice with ice-cold phosphate-buffered saline (PBS), and then incubated at 55°C overnight with digestion buffer (100 mM NaCl, 25 mM EDTA, 10 mM Tris-HCl [pH 8.0], 0.5% sodium dodecyl sulfate, 0.1 mg/ml proteinase K).

In brief, the cells were washed with 5 ml ice-cold PBS-PMSF after BD treatment.