In brief, cells were trypsinized, suspended in serum-free DMEM and brought to 10 cells/ml for all cell lines.
In brief, cells were trypsinized, and 20,000 cells/sample were mixed with the same volume of the Apo-one Homogenous Caspase-3/7 reagent.
In brief, cells were trypsinized, stained with LIVE/DEAD fixable violet (Invitrogen), fixed with 2% paraformaldehyde, and permeabilized with 0.3% saponin.
In brief, cells were trypsinized and re-suspended at 5 × 10/ml in room temperature phenol-red-free Leibowitz L15 medium/10% (vol/vol) FCS plus 0.1 mmol/l sulfinpyrazone (Sigma).
In brief, cells were trypsinized and lysed with modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP40, 0.25% Na-deoxycholate, 1 mM EDTA) containing protease inhibitors (1 μg/ml aprotinin, 1 μgml leupeptin, 1 μg/ml pepstatin, 1 mM PMSF, 1 mM sodium orthovanadate and 1 mM sodium fluoride) for 30 min on ice.
At confluence, cells were trypsinized with trypsin-EDTA for 5 min to detach the cells from the culture substrates.
The cells were trypsinized with trypsin-EDTA.
In brief, the cells (1 × 10 cells) were trypsinized and resuspended with 1 mL complete medium and added onto the polymerized Matrigel (BD Biosciences).
Cells were trypsinized with 0.25% trypsin-EDTA.
