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Exact(25)
In brief, cells were plated in 96 well plates and transfected with siRNA from the library.
In brief, cells were plated onto poly-L-ornithine-coated glass coverslips and fixed with 4% paraformaldehyde for 15 to 20 minutes at room temperature, and then were washed twice (10 minutes each) with 1× PBS.
In brief, cells were plated in duplicate into 6-well microplates at 5×106 cells/well, and incubated in drug-free medium or medium containing 17-AAG, or 17-AAG plus cisplatin of varying concentrations at 37°C for 24 h.
In brief, cells were plated in quadruplicates in 24-well plates under standard conditions.
In brief, cells were plated at a density of 1.8 × 10 cells.
In brief: cells were plated at 50% confluence and starved overnight (MEM, 0.2% FBS).
Similar(35)
In brief, cell were plated in chamber well slides and cultured in Dulbecco's Minimum Essential Medium supplemented with 10% fetal bovine serum until confluent.
In brief, 2000 cells were plated per well of a 96-well plate and treated 24 h later [23].
In brief, 804G cells were plated onto 100 mm dishes or 96 well plates and allowed to reach confluence.
In brief, 3×106 cells were plated onto 10 cm cell culture dishes and after 24 hrs treated as indicated.
In brief, 2×105 Cells were plated in a 6-well plate and treated for 4 h with WFA (0.5 µM) or DMSO.
More suggestions(15)
brief cells were dissociated
brief cells were passaged
brief cells were grown
brief cells were pelleted
brief cells were transfected
brief monocytes were plated
brief cells were fixed
brief cells were spread
brief cells were seeded
brief cells were labeled
brief cells were flushed
brief cells were resuspended
brief cells were incubated
brief cells were maintained
brief cells were stained
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