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Exact(23)
In brief, cells were grown at 32 °C in Edinburgh Minimal Medium (EMM) supplemented with 0.25 mg/ml uracil, to induce Purg1.
In brief, cells were grown, cross-linked with formaldehyde and sheared by sonication.
In brief, cells were grown in liquid culture overnight at 30°C and 37°C.
In brief, cells were grown on Matrigel-coated plates in mouse embryonic fibroblast-conditioned medium (MEF-CM) supplemented with basic fibroblast growth factor (bFGF) at a concentration of 8 ng/mL.
In brief, cells were grown in SILAC RPMI 1640 Medium (Pierce Biotechnology, Rockford, USA) containing 10% v/v dialyzed FBS, and either 0.1 mg/mL heavy [13C6] or light [12C6] L-lysine (Pierce Biotechnology, Rockford, USA).
In brief, cells were grown on glass coverslips coated with collagen.
Similar(37)
In brief, EAhy926 cells were grown to about 60% confluence in collagen coated cover slips.
In brief, the cells were grown to confluence, detached, transferred to tubes and pelleted by centrifugation.
In brief, yeast cells were grown in YPD at 27°C and harvested at mid-log phase (OD = 0.6).
In brief, AB8/13 cells were grown in standard RPMI 1640 medium containing 10% fetal calf serum and supplements either at the permissive temperature of 33 °C (in 5% CO2) to promote cell propagation or at the non-permissive temperature of 37 °C (in 5% CO2) to allow the terminal differentiation.
In brief, the cells were grown at 37°C in 5% CO2 in complete ES medium: DMEM (Invitrogen), 15% heat inactivated fetal bovine serum (FBS) (Life Technologies), 1000 U ml−1 leukaemia inhibitory factor (ESGRO), 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 2 mM GlutaMAX, 0.1 mM β-mercaptoethanol, and penicillin/streptomycin (50 U/50 mg ml−1).
More suggestions(15)
brief cells were dissociated
brief cells were passaged
brief cells were washed
brief cells were discriminated
brief cells were pelleted
brief cells were lysed
brief cells were fixed
brief cells were seeded
brief cells were spread
brief cells were labeled
brief cultures were grown
brief cells were flushed
brief cells were resuspended
brief cells were maintained
brief cells were stained
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