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In brief, cells were flushed out of leuko-reduction filters with 40 ml PBS without Ca2+ and Mg2+ using a syringe.
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In brief, bone marrow cells were flushed out of femur and tibia bones with PBS.
In brief, the bone marrow cells were flushed from the long bone collected and prepared for single cells suspension.
Tibia and femur were extracted aseptically and the bone marrow (BM) cells were flushed from tibia and femur of mice.
Bone marrow cells were flushed and isolated as previously described.
After three washes, the column was removed from magnetic filed and CD11b+ cells were flushed out.
Cells were flushed out using sterile PBS and collected in 15 ml Falcon tubes.
BM cells were flushed from tibias and femurs with PBS.
Bone marrow cells were flushed from tibiae and femurs of C57BL/6 mice.
Marrow cells were flushed from the femurs with a syringe containing 2 mL PBS-1% FBS.
Mouse bone marrow cells were flushed from femur using α-MEM.
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