In brief, cells were fixed by adding formaldehyde to 1% final concentration for 10 min at room temperature.
In brief, cells were fixed with 4% formaldehyde in PBS for 30 min. Following fixation, cells were treated with 0.2% Triton X-100 in PBS for 5 min. After blocking with 10% FBS in PBS for 20 min, cells were incubated for 1 h with primary antibody, followed by washing in PBS and incubation for 1 h with the relevant secondary antibody.
In brief, cells were fixed with 2% paraformaldehyde, permeabilized with the addition of 0.1% Triton X-100 in phosphate-buffered saline (PBS) for 10 minutes, quenched in an isotonic solution of 50 mM glycine in diluted PBS for 10 minutes, and then blocked with 1.5% normal goat serum in PBS at 4° C overnight.
In brief, cells were fixed in 4% formaldehyde, blocked in 5% bovine serum albumin (BSA) in phosphate buffered saline (PBS), incubated with primary anti-PLAP (8B6) diluted 1∶1000 in 1% BSA in PBS for 2 hrs at RT, washed in PBS, incubated with Alexa Fluor® 488-donkey anti-mouse IgG 1∶2000 and DAPI (1 ng/µl) in 1% BSA in PBS for 1 hr at RT, and washed in PBS.
In brief, cells were fixed for 30 min at room temperature using 4% buffered paraformaldehyde.
In brief, cells were fixed with 1% formaldehyde in PBS to crosslink chromatin.
In brief, labeled cells were fixed with 4% paraformaldehyde and images obtained with a Leica DM IRE2 microscope [66], [67].
In brief, 2×107 cells were fixed with 1% paraformaldehyde for 10 min. Cross-linking was stopped by the addition of 125 mM glycine, and chromatin derived from isolated nuclei was sheared using a F550 micro-tip cell sonicator (Fisher Scientific).
In brief, the cells were fixed with paraformaldehyde, and cells were permeabilized with Triton-X 100.
In brief, the cells were fixed with 4% PFA for 10 min.
In brief, the cells were fixed in 4% paraformaldehyde for 10 min and stained overnight at room temperature.
