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In brief, cells were collected by scraping cells into ice-cold TBS and centrifuging at 14000×g for 10 min at 4°C.
In brief, cells were collected from YPAD medium by filtration, and the cell paste was frozen with EM PACT2 high pressure freezer (Leica, Austria).
In brief, cells were collected by centrifugation at 4°C and cell pellets were resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40, 0.15 mM spermine, 0.5 mM spermidine).
In brief, cells were collected by centrifugation and washed with 0.05 mol/L of PBS.
In brief, cells were collected, washed with PBS and stained according to the manufacturer's protocol.
In brief, cells were collected by trypsinization after transfection with either control vector or pMS2-lincRNA-p21 plasmids and stained with 0.4% trypan blue reagent (Sigma).
Similar(51)
In brief, the cells were collected and washed three times with 10% glycerol reducing the volume each time.
In brief, floating cells were collected together with the supernatant and adherent cells were harvested by trypsinisation.
In brief, cervical cells were collected with a cervical sampler (Digene Corporation, Gaithersburg, MD, USA) using the cervical cytobrush technique, and were divided into three parts.
In brief, Xoo cells were collected from 4 ml suspension by centrifuging at 12,000 × g for 5 min and mixed with 1 ml absolute methanol.
In brief, Raji cells were collected and suspended at 2 × 10/mL in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS).
More suggestions(15)
brief cells were lysed
brief nuclei were collected
brief cells were resuspended
brief bacteria were collected
brief cells were seeded
brief cells were cultured
brief pellets were collected
brief images were collected
brief cells were dissociated
brief spores were collected
brief cells were exposed
brief samples were collected
brief cells were maintained
brief cells were spread
brief cells were stained
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