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In brief, cells are seeded on fibronectin-coated 96-well plates, covered with a monolayer of carboxylated latex beads.
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In brief, cells were grown at 32 °C in Edinburgh Minimal Medium (EMM) supplemented with 0.25 mg/ml uracil, to induce Purg1.
In brief, cells were seeded (20,000 cells per well) in the 96-well plates.
In brief, cells were loaded with 1 μmol/L DHE for 30 min at 37°C.
In brief, cells were transfected with Flag-tagged TFEB-WT or TFEB-K116R for 24 h.
In brief, cells were seeded in 96-well plates at the density of 1 × 104/well.
In brief, cells were seeded into 60 mm petri dishes at the density of 3.0 × 104 cells/cm2.
In brief, cells were seeded in six-well tissue culture plates at a density of 1 × 105 cells per well.
In brief, cells were seeded in 96-well plates.
In brief, cells were infected with LC3-GFP adenovirus.
In brief: Cells were harvested and lysed, and the chromosomal DNA was isolated and purified.
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