Sentence examples for brief cells and from inspiring English sources

In brief, cells and EVs were lysed in RIPA buffer containing a cocktail of protease inhibitors and the quantification was performed using Bradford.

In brief: Cells were harvested and lysed, and the chromosomal DNA was isolated and purified.

In brief, cells were harvested and collected following trypsin detachment and centrifugation.

In brief, cells were harvested and washed twice in PBS, and lysed in a lysis buffer (Pierce) supplemented with protease inhibitor mixture (Sigma).

In brief, cells were harvested and lysed with a buffer containing digitonin (80  μg/ml) and centrifuged at 500 × g to remove cell debris.

In brief, cells were treated and stained with 2 μM DCFH-DA for 30 minutes and fluorescence was measured using an argon ion laser at 488 nm excitation and 510 to 540 nm emission on a FACscan flow cytometer (Becton Dickinson).

In brief, cells were harvested and homogenized in buffer containing 250 mM sucrose, 10 mM Tris and 1 mM EDTA, pH 7.4.

In brief, cells were resuspended and treated with a solution of 0.1% Triton X-100 and 0.1% sodium citrate for 5 min at room temperature, then pelleted and washed twice with PBS for 5 min each.

In brief, cells were washed and then scraped in cold PBS supplemented with phosphatase inhibitors.

In brief, cells were washed and incubated for 45 min at 37°C with MEM containing 0.5% glucose.

In brief, cells were lysed and the proteins (20 µg) were incubated with the Csp-12 substrate ATAD-AFC (50 µg) or with the Csp-3 substrate DEVAD-AFC (50 µg), for 2 hours at 37°C.