Sentence examples for brief cell from inspiring English sources

In brief, cell wall samples were lyophilized overnight and incubated with 48% hydrofluoric acid for 48 h to remove secondary cell wall polymers.

In brief, cell pellets were resuspended in a buffer containing 50 mM HEPES (pH 7.8), 300 mM NaCl, 5% glycerol, 20 mM imidazole and 0.5 mM TCEP, and lysed using an emulsiflex-C3 high pressure homogenizer (Avestin).

In brief, cell culture supernatants were added to cytokine specific antibody-coated wells, followed by the addition of a biotinylated detection antibody.

In brief, cell were plated in chamber well slides and cultured in Dulbecco's Minimum Essential Medium supplemented with 10% fetal bovine serum until confluent.

In brief, cell pellets were resuspended in Lysis 250 buffer followed by a freeze and thaw process that was performed 3 times.

In brief, cell lysis was performed using cell lysis buffer (Cell Signaling, Frankfurt a.M., Germany) supplemented with Complete protease inhibitor cocktail.

In brief, cell-free supernatant was acidified to precipitate rhamnolipids by addition of 6 N HCl to pH 2 and kept at room temperature overnight.

In brief, cells were seeded (20,000 cells per well) in the 96-well plates.

In brief, cells were loaded with 1 μmol/L DHE for 30 min at 37°C.

In brief, cells were transfected with Flag-tagged TFEB-WT or TFEB-K116R for 24 h.

In brief, cells were seeded in 96-well plates at the density of 1 × 104/well.