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In brief, brain samples were obtained at the Allegheny County Medical Examiner's Office (Pittsburgh) after written consent from next-of-kin.
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In brief, hippocampal brain samples were cut into 350 µm thick horizontal slices on a vibratome (Leica Microsystems, Wetzlar, Germany).
Rat brain samples were homogenized in the same buffer used for the human brain samples.
The homogenized brain samples were imaged under 60x magnification.
Brain samples were processed as described in detail before51.
Brain samples were serially sectioned at the striatal region.
The brain samples were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl).nl
Western blot analyses of brain samples were performed as described in33.
Brain samples were collected after 1 week.
†No blood or brain samples were available.
Brain samples were shipped on dry ice.
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