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In brief, bone marrow cells were flushed out of femur and tibia bones with PBS.
In brief, bone marrow cells were isolated by crushing iliac crest bones, femurae, and tibiae in PBS containing 0.2% BSA and 2 mM EDTA.
In brief, bone marrow cells were isolated from female C57Bl/6J mice (Jackson Laboratories) and cultured in RPMI based media (Lonza) supplemented with 10% heat inactivated FBS (Sigma-Aldrich), 1% penicillin/streptomycin, 50 μM β-mercaptoethanol, and 20 ng/mL GM-CSF (Peprotech).
In brief, bone marrow cells were harvested from the femurs and tibias of mice and plated in RPMI-1640 medium supplemented with 10% FBS, 50 μM 2-mercaptoethanol, and high-dose (200 U/ml) murine GM-CSF (Endogen, Inc., Cambridge, MA, USA).
Similar(56)
In brief, the bone marrow cells were flushed from the long bone collected and prepared for single cells suspension.
In brief, 40×106 unfractionated CFSE-labeled bone marrow cells were i.v. injected into recipients and the numbers of CFSE-labeled cells in thymi were determined 24 hours later by flow cytometry.
Bone marrow cells were extracted, and erythrocytes were removed by brief exposure to 0.168 M NH4Cl.
Osteogenesis in transplants of bone marrow cells.
Bone marrow cells are a possible candidate for this approach.
Primary bone marrow cells were grown in "C10" media49.
Orlic, D. et al. Bone marrow cells regenerate infarcted myocardium.
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